BIOFILMS AND HARD-TO-HEAL WOUNDS
Ajoene: First in Class Quorum Sensing Inhibitor that Prevents Biofilm Formation, the Production of Virulence Factors and Modulates Established Biofilms
D.M.J. Houston, D. Neef, P. Rice, D. Williams, H. Taleb, L. Jones, L. Andrews, J. Preece, L. Sykes, W.W. Nichols, G. Dixon.
NX-AS-911 modulates quorum sensing to attenuate P. aeruginosa and S. aureus biofilms and virulence
D. Neef, D. Williams, H. Taleb, M. Williams, J. Preece, A. Bernard, W. Nichols, G. Dixon
Breaking the barrier: a novel approach to controlling infection in hard-to-heal wounds
Welsh Wound Innovation centre (WWIC), UK¹; Neem Biotech Ltd., UK²; Miller School of Medicine (University of Miami), U.S.A³
Harding, Keith¹; Gabbay, Flic²; Barnard, A²; Taleb, H²; Neef, D²; Head, C³, Pastar, I³ and Dixon, G²
SOME OF THE WORK WE HAVE BEEN INVOLVED IN
Technological platform to develop nutritional additives to reduce methane emissions from ruminants (SMEthane)
Yáñez-Ruiz, D.R. (2012)
Agencia Estatal Consejo Superior de Investigaciones Cientificas, Spain
Ruminant nutrition regimes to reduce methane & nitrogen emissions
Newbold, J. (2010)
Aberystwyth University
SOME OF OUR PEER-REVIEWED PUBLICATIONS
Synthetic Chemistry
Short Total Synthesis of Ajoene
Silva, F., Khokar, S., Williams, D.M., Saundres, R., Evans, G.J.S., Graz, M. and Wirth, T. (2018)
Angew.Chem.Int.Ed. 10.1002/anie.201808605
Flow Alkylation of Thiols, phenols and Amines Using a heterogenous Base in a packed-Bed Reactor
Baker, A., Graz, M., Saunders, R., Evans, G., Piotti, I. and Wirth, T. (2015)
J.Flow.Chem, 5(2):65-68.
Flow Synthesis of Symmetrical di- and Trisulfides using phase-Transfer Catalysis
Baker, A., Graz, M., Saunders, R., Evans, G., Kaul, S. and Wirth, T. (2013)
J. Flow Chem, 3:118-121
Veterinary interventions
Disrupted intracellular redox balance of the diplomonad fish parasite Spironucleus vortens by 5-nitroimidazoles and garlic-derived compounds
Williams, C., Lloyd, D., Kolarich, D., Alagesan, K., Duchêne, M., Cable, J., Williams, dD. and Leitsch, D. (2012)
Veterinary parasitology: 190(1-2):62-73
Effect of garlic and allium-derived products on the growth and metabolism of Spironucleus vortens
Milet, C.O., Lloyd, D., Williams, C., Williams, D., Evans, G., Saunders, R. and Cable,. J. (2011)
Experimental parasitology: 127(2):490-9
Rumen microbiology
Plant extracts to manipulate rumen fermentation
Hart, K., Yáñez-Ruiz, D.R., Duval, S.M., Mcewan, N.R. and Newbold, C.J. (2008)
Anim. Feed Sci.Technol, 11, 147 (1-3), doi:10.1016/j.anifeedsci.2007.09.007.
Commercial considerations
Exploring new business models: Rising to the challenge of antibiotic development
Graz, H. (2019)
MedNous, 13(8): 8-10.
SOME OF OUR CONFERENCE PRESENTATIONS
Assay development for Quorum Sensing
Development of an in-house in vitro Quorum Sensing Inhibitor detection assay and confirmation of the Quorum Sensing Inhibition activity of NX-AS-401,
Williams, D., Oldham, H. and Graz, M. (2017)
40th European Cystic Fibrosis Conference, Seville, Spain (Poster)
Bacterial lung infections in cystic fibrosis
NX-AS-401 Enhances antiobiotic effects on multiple relevant bacteria
Sykes, Lucy; Taleb, Hajer; Williams, Danielle; Preece, J; Clarke, Jenny; Neef, Daniel; Oldham, H; Kadioglu, Aras; Dixon, Graham
Neem Biotech NACFC 2018 (Poster)
NX-AS-401: A Quorum sensing inhibitor that reduces virulence factor production, disrupts established biofilms and enhances the effect of tobramycin in multiple clinical isolates
Sykes, Lucy; Williams, Danielle; Preece, J; Taleb, H; Barnard, A; Oldham, H; Graz, M and Dixon, G.
ECFS Basic Science 2018 conference (Poster)
Exploring economic value in treating Pseudomonas aeruginosa infections in cystic fibrosis
Graz, M., Walker, S., Alderson, D., Morris, J. and Graz, H. (2017)
– early modeling to inform product development”, 22nd Annual ISPOR Congress, Boston, USA (Poster)
NX-AS-401: A quorum sensing inhibitor that enhances the effect of P.aeruginosa in vitro and in vivo
Williams, D., Sykes, L., Bricio-Moreno, L., Fothergill, J., Kadioglu, A., Oldham, H., Clark, S. and Graz, M. (2017)
North American Cystic Fibrosis Conference, Indianapolis (poster)
Potential therapeutic uses of novel antimicrobial peptides isolated from the rumen microbiome against Pseudomonas aeruginosa,
Mulkern, A., Oyama, L, Graz, M., Evans, G., Williams, D. and Huws, S. (2017)
SDRI Conference, Brisbane, Australia (Poster)
Ajoene, a novel therapeutic that shows enhanced activity in in vivo and in vitro models of Pseudomonas aeruginosa infection,
Bricio-Morena, L., Waters, B., Neil, D., Evans, G., Graz, M., Fothergill, J. and Kadioglu, A. (2017)
SDRI Conference, Brisbane, Australia (Poster)
Effect of (E,Z)-4,5,9-trithiadodeca-1,6,11-triene 9-oxide in in vivo and in vitro Models of Pseudomonas aeruginosa Infection
Graz, M., Williams, D., Bricio-Moreno, L., Fothergill, J. and Kadioglu, A. (2017)
ASM/ESCMID, Boston, MA (Poster)
Payer perspectives on the requirements for a novel inhaled combination to improve management of Pseudomonas aeruginosa infections in cystic fibrosis patients – What is needed?
Graz, H., Alderson, D., Morris, J. and Graz, M. (2017)
22nd Annual ISPOR Meeting, Glasgow, UK (poster)
Antimicrobial effects of plant extracts
Bioaccessability of organosulphur conpounds from Allium sativum
Martí, G., Evans, G., Jones, L., Viadel, B., Ruiz, B., Gallego, E. and Graz, M. (2017)
ICMAN-IUPHAR, Aberdeen, UK (Poster)
Identification of a novel synergistic combination of natural extracts for infection control in diabetic ulcers
Whatmough, A., Williams, D., Evans, G. and Graz, M. (2017)
ICMAN-IUPHAR, Aberdeen, UK (Poster)
Antimicrobial Effects of a Proprietary Standardised Garlic Extract iQ-AS-105: A broad spectrum activity on Gram Negative, Gram Positive and Fungal Microorganisms
Peng, L.V., De Costa P., Chong P.W., Graz M. and Jee J.M. (2014)
Proceedings of the Antibiotic Alternatives for the New millennium, London, UK. (Poster)
Inhibitory Effects of Garlic Metabolites on Clostridium difficile
Williams R.O., Day, M., Lloyd, D., Wirth, T., Jones, L., Evans, G., De Costa, P. and Graz, M. (2014)
Proceedings of the Antibiotic Alternatives for the New millennium, London, UK. (Poster)
Synthetic chemistry
Synthesis of Di- and Trisulfides using Flow Reactors
Baker, A., Graz, M., Saunders, R., Evans, G. and Wirth, T. (2013)
Presented at the 12th International Conference on Selenium and Tellurium, Cardiff, UK (poster)
Synthesis of Di- and Trisulfides using Flow Reactors
Baker, A., Graz, M., Saunders, R., Evans, G. and Wirth, T. (2013)
Presented at the 2nd SCi/RSC Symposium on Continuous Processing and Flow Chemistry, Horsham, UK
Bioanalysis
Thabit Jones, A., Graz, M and Oldham, H (2017)
Quantitative analysis of an oil based natural compound extracted from plasma
ICMAN-IUPHAR, Aberdeen, UK (Poster and oral presentation)
Drug development studies
Drug Development with natural products – A journey
Oldham, H. (2017)
ICMAN-IUPHAR, Aberdeen, UK
Drug metabolism studies with natural products – A journey
Oldham, H. (2017)
46th Drug Metabolism Discussion Group Open Meeting, Cambridge, UK
P1b - Drug metabolism studies with natural products
Oldham, H. (2017)
46th Drug Metabolism Discussion Group Open Meeting, Cambridge, UK
Mootral
The effect of a blend of natural compounds (NX-RH-201) on the quality of milk produced on a commercial farm under normal operational conditions
Graz, M., Vrancken, H., Riede, O. and Brandsma, M. (2017)
International Dairy Federation Summit, Ireland (poster)
OUR PATENTS
Process for the preparation of Ajoene (Patent WO 2010100486 A 3) 2010
Process for the production of Allicin (Patent WO 2003004668 A 1) 2003
Extraction method for Azadirachtin (Patent WO 2002032907 A 1) 2002